Activity of phospholipase A2
نویسندگان
چکیده
منابع مشابه
Assaying phospholipase A2 activity.
Mammalian cells contain many structurally and functionally diverse phospholipases A2 (PLA2) that catalyze the hydrolysis of sn-2 fatty acid from membrane phospholipid. Assays are described for measuring the activity of Group IVA cytosolic PLA2alpha(cPLAalpha) and for secreted PLA2s (sPLA2) that are suitable for purified enzymes and for measuring activity in crude cell lysates and culture medium...
متن کاملInhibition of phospholipase A2.
Phospholipases A2 are involved in inflammatory processes such as the liberation of free arachidonic acid from the membrane pool for the biosynthesis of eicosanoids. Inhibitors of these enzymes are proving useful in determining the biological roles of phospholipases A2 in complex cellular processes and may also have therapeutic potential. Inhibition of these lipolytic enzymes is more difficult t...
متن کاملCharacterization of Ca2+-dependent phospholipase A2 activity during zebrafish embryogenesis.
We have developed a simple fluorescent assay for detection of phospholipase A2 (PLA2) activity in zebrafish embryos that utilizes a fluorescent phosphatidylcholine substrate. By using this assay in conjunction with selective PLA2 inhibitors and Western blot analysis, we identified the principal activity in zebrafish embryogenesis as characteristic of the Ca2+-dependent cytosolic PLA2 (cPLA2) su...
متن کاملEnzymatic Activity of a Phospholipase A2: An Electrochemical Approach
A novel approach based on dc and ac cyclic voltammetric techniques to evaluate interfacial enzymatic activity is presented. As an illustrative example, the hydrolytic effect of phospholipase A2 (PLA2) on an L-R-dioleoylphosphatidylcholine (DOPC) monolayer adsorbed onto a mercury electrode surface has been studied and kinetic parameters have been evaluated. The enzymatic activity obtained in thi...
متن کاملMaintenance of Synaptic Stability Requires Calcium-Independent Phospholipase A2 Activity
Phospholipases A₂ (PLA₂s) represent one of the largest groups of lipid-modifying enzymes. Over the years, significant advances have been made in understanding their potential physiological and pathological functions. Depending on their calcium requirement for activation, PLA₂s are classified into calcium dependent and independent. This paper mainly focuses on brain calcium-independent PLA₂ (iPL...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Gut
سال: 1988
ISSN: 0017-5749
DOI: 10.1136/gut.29.5.693-a